麻豆果冻传媒

For Release: September 26, 2012

STANFORD, Calif. 鈥� Healthy people鈥檚 lungs are home to a diverse community of microbes that differs markedly from the bacteria found in the lungs of cystic fibrosis patients. That鈥檚 the result of new research from Stanford University and Lucile Packard Children鈥檚 Hospital Stanford, which has wide implications for treatment of cystic fibrosis and other lung diseases.

鈥淭he lung is not a sterile organ,鈥� said聽David Cornfield, MD, an author of the new study, which will be published Sept. 26 in Science Translational Medicine. Although decades of received scientific wisdom said healthy lungs lacked resident microbes, scientists had begun questioning that notion. 鈥淭his research confirmed a long-held suspicion that a forest of microbes exists in both healthy and diseased lungs,鈥� said Cornfield, a pulmonologist at Packard Children鈥檚 and a professor of pediatrics in pulmonary medicine at the School of Medicine. 鈥淢ore surprising, our data presents a suggestion that the lung flora provides microbial homeostasis that might function to preserve health.鈥� Healthy lungs鈥� microbes have been overlooked in part because past research has focused heavily on lung diseases, Cornfield said. Another flaw in prior studies was a bias toward looking for micro-organisms that could be grown in labs. Many of the types of microbes that the Stanford researchers found in healthy lungs have never been cultured in a laboratory.

In contrast, a large body of research had previously shown chronic microbial colonization in the lungs of people with cystic fibrosis, a genetic disease characterized by serious, progressive lung problems and death from respiratory failure. For instance, CF patients are vulnerable to chronic infection with the bacteria Pseudomonas aeruginosa, which can cause pneumonia. The new study used sputum samples from 16 CF patients and nine healthy control patients. The scientists also analyzed lung tissue samples from seven patients鈥� explanted lungs, the organs removed when the patients received lung transplants. (Three lung-transplant recipients had CF; four had other lung diseases.) The researchers extracted DNA from the sputum and tissue, and selectively copied genes coding for the 16S ribosomal gene sequences, which are found only in bacteria. The resulting genetic material was measured to determine which phyla, or families, of bacteria it came from and the relative contributions of each bacterial phylum to the total bacterial population in the lungs.

Several differences emerged between CF patients and healthy people鈥檚 communities of lung bacteria. In general, healthy individuals had more diversity among their lung bacteria. Different bacterial phyla predominated in the two groups: members of the Bacteroidetes and Fusobacteria phyla were much more prominent in healthy individuals, whereas CF patients had a larger percentage of Actinobacteria. Also, healthy people had a larger proportion of bacteria that had never been grown in a lab. 鈥淚 think the tendency toward decreased diversity can be metaphorically viewed as the same phenomenon that might happen in a rainforest,鈥� Cornfield said. 鈥淲hen the ecosystem of a rainforest is disturbed and one organism predominates, it undermines a carefully constructed balance and causes disturbances in overall ecosystem. I think it鈥檚 reasonable to assume something similar could happen in the lung microbiome, where pathogenic bacteria may out-compete organisms that may play a salutary, health-affirming role.鈥�

The results open many questions for future research. No one has ever tested the idea that certain microbes benefit lung health, for instance. 鈥淲e may need to consider strategies that allow favorable microbes to exist while eradicating disease-causing species,鈥� Cornfield said. 鈥淭hat paradigm, if it鈥檚 true, would really turn the care of patients with pneumonia and other lower-airway diseases on its head.鈥� Future research might test whether CF or pneumonia patients could benefit from doses of probiotic bacteria to their lungs, he said. In addition, no one is sure how the antibiotics often given to CF patients change the microbes in their lungs.

鈥淭he marked differences in composition and diversity of microbial communities from adults with cystic fibrosis and normal controls are intriguing,鈥� said Thomas Ferkol, MD, director of allergy, immunology and pulmonary medicine at the Washington University School of Medicine in St. Louis. 鈥淭he question that remains to be answered is whether these differences are directly related to the underlying lung disease or simply a consequence of frequent antibiotic use, which has been shown to change microbiota of the upper airways.鈥� Ferkol was not involved in the research. More questions arise from the fact that bacterial profiles varied within the group of CF patients. CF patients differ widely in their disease progression, even when they have the same disease-causing gene mutations. It is possible that patients鈥� lung function may be linked to the bacteria present in their lungs. The research team now plans to study whether individual patients鈥� bacterial profiles can be used to predict their clinical condition.

Cornfield collaborated with first author Paul Blainey, PhD, previously a postdoctoral scholar at Stanford and now a faculty member at the Massachusetts Institute of Technology; Carlos Milla, MD, an associate professor of pediatrics in pulmonary medicine at Stanford and a pulmonologist at Packard Children鈥檚; and senior author Stephen Quake, PhD, professor of bioengineering and of applied physics at Stanford and an investigator at the Howard Hughes Medical Institute. The Department of Bioengineering is jointly operated by Stanford鈥檚 school of Medicine and of Engineering. The research was funded by Quake鈥檚 HHMI funds and by grants from the National Institutes of Health, including an NIH Director鈥檚 Pioneer Award.

More information on the Department of Pediatrics, which also supported the research, is available at .

Authors

Erin Digitale
digitale@stanford.edu